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Rath, Archana
- Metagenomic Analysis of Total Microbial Diversity and Antibiotic Resistance of Culturable Microorganisms in Raw Chicken Meat and Mung Sprouts (Phaseolus aureus) Sold in Retail Markets of Mumbai, India
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Authors
Affiliations
1 Department of Biotechnology, University of Mumbai, Vidyanagari, Santacruz (East), Mumbai 400 098, IN
2 Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, IN
3 Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, IN
1 Department of Biotechnology, University of Mumbai, Vidyanagari, Santacruz (East), Mumbai 400 098, IN
2 Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, IN
3 Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, IN
Source
Current Science, Vol 113, No 01 (2017), Pagination: 71-79Abstract
Raw chicken meat and ready-to-eat sprouts are potential sources of food-borne infections. Development and spread of antibiotic resistance (AR) in microflora associated with food is a major health concern. In this study, we employed culturable and non-culturable methods to characterize microflora associated with chicken meat and mung. Pathogens belonging to Enterobacteriaceae were dominant in the culturable set. Rare species like Citrobacter amalonaticus, Kluyvera georgiana, Kurthia gibsonii and Staphylococcus hominis were isolated and metagenomic study revealed overall good species richness in both food types, Firmicutes and Gammaproteobacteria were dominant phyla in chicken meat and sprouts respectively. Common food-borne and opportunistic pathogens like Campylobacter, C. perfringens, Streptococcus, Shewanella, Pseudomonas, Aeromonas hydrophila, Staphylococcus, E. coli, Acinetobacter, Enterobacter, Klebsiella were detected and 18% of the genera were common to both food types. We observed high AR bacterial count (5 to 9 log CFU/g) in the microflora. Fifty AR isolates per food type were identified with high multiple AR index of 0.3-0.9.Keywords
Chicken Meat, Food-Borne Pathogens, Metagenomics, Multiple Antibiotic Resistance, Mung Sprouts.Full Text
References
- Fratamico, P. M., Bhunia, A. K. and Smith, J. L., In Foodborne Pathogens: Microbiology and Molecular Biology, Caister Academic Press, Wymondham, Norfolk, UK, 2005, p. 273. ISBN: 1-9044550-00-X.
- Yildirim, Y., Gonulalan, Z., Pamuk, S. and Ertas, N., Incidence and antibiotic resistance of Salmonella spp. on raw chicken carcasses. Food Res. Int., 2011, 44, 725–728.
- Hanning, I. B., Nutt, J. D. and Ricke, S. C., Salmonellosis outbreaks in the United States due to fresh produce: sources and potential intervention measures. Foodborne Pathog. Dis., 2009, 6, 635–648.
- Bayer, C. et al., An outbreak of Salmonella Newport associated with mung bean sprouts in Germany and the Netherlands, October to November 2011. Euro. Surveill., 2014, 19, pii=20665.
- Taormina, P. J., Beuchat, L. R. and Slutsker, L., Infections associated with eating seed sprouts: an international concern. Emerg. Infect. Dis., 1999, 5, 626–634.
- Harris, L. J., Farber, J. N., Beuchat, L. R., Parish, M. E., Suslow, T. V., Garrett, E. H. and Busta, F. F., Outbreaks associated with fresh produce: incidence, growth, and survival of pathogens in fresh and fresh-cut produce. Compr. Rev. Food Sci. F., 2003, 2, 78–141.
- Viswanathan, P. and Kaur, R., Prevalence and growth of pathogens on salad vegetables, fruits and sprouts. Int. J. Hyg. Environ. Health, 2001, 203, 205–213.
- Boehme, S., Werner, G., Klare, I., Reissbrodt, R. and Witte, W., Occurrence of antibiotic-resistant enterobacteria in agricultural foodstuffs. Mol. Nutr. Food Res., 2004, 48, 522–531.
- Ali, N. H., Farooqui, A., Khan, A., Khan, A. Y. and Kazmi, S. U., Microbial contamination of raw meat and its environment in retail shops in Karachi, Pakistan. J. Infect. Dev. Ctries., 2010, 4, 382–388.
- Yates, C. M., Shaw, D. J., Roe, A. J., Woolhouse, M. E. and Amyes, S. G., Enhancement of bacterial competitive fitness by apramycin resistance plasmids from non-pathogenic Escherichia coli. Biol. Lett., 2006, 2, 463–465.
- Choudhury, R., Panda, S. and Singh, D. V., Emergence and dissemination of antibiotic resistance: a global problem. Indian J. Med. Microbiol., 2012, 30, 384–390.
- Simon, C. and Daniel, R., Metagenomic analyses: past and future trends. Appl. Environ. Microbiol., 2011, 77, 1153–1161.
- Mehndiratta, P. L. and Bhalla, P., Use of antibiotics in animal agriculture and emergence of methicillin-resistant Staphylococcus aureus (MRSA) clones: need to assess the impact on public health. Indian J. Med. Res., 2014, 140, 339–344.
- Grace, D., Review of evidence on antimicrobial resistance and animal agriculture in developing countries. Review report, International Livestock Research Institute (ILRI), Nairobi, Kenya, 2015.
- Schwaiger, K., Huther, S., Hölzel, C., Kämpf, P. and Bauer, J., Prevalence of antibiotic-resistant enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany. Int. J. Food Microbiol., 2012, 154, 206–211.
- Kumaraswamy, K. K. et al., Emergence of a new antibiotic resistance mechanism in India, Pakistan and the UK: a molecular, biological and epidemiological study. Lancet Infect. Dis., 2010, 10, 597–602.
- deSousa, C. P., The impact of food manufacturing practices on food-borne diseases. Braz. Arch. Biol. Technol., 2008, 51, 815–823.
- Berger, C. N., Sodha, S. V., Shaw, R. K., Griffin, P. M., Pink, D., Hand, P. and Frankel, G., Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ. Microbiol., 2010, 12, 2385–2397.
- Bywater, R. et al., A European survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from foodproducing animals. J. Antimicrob. Chemoth., 2004, 54, 744–754.
- Bauer, A. W., Kirby, W. M. M., Sherris, J. G. and Turk, A., Antimicrobial susceptibility testing by standardized single disc method. Am. J. Clin. Pathol., 45, 493–496.
- Clinical and Laboratory Standards Institute (CLSI), Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, Approved Standard – Third Edition (M31-A3), 2008.
- European Committee on Antimicrobial Susceptibility Testing (EUCAST), Breakpoint tables for interpretation of MICs and zone diameters, version 5.0; http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_5.0_Breakpoint_Table_01.pdf, 2015.
- Carlson, S. A., Bolton, L. F., Briggs, C. E., Hurd, H. S., Sharma, V. K., Fedorka-Cray, P. J. and Jones, B. D., Detection of multiresistant Salmonella typhimurium DT104 using multiplex and fluorogenic PCR. Mol. Cell. Probe., 1999, 13, 213–222.
- Nossa, C. W. et al., Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J. Gastroenterol., 2010, 16, 4135–4144.
- Lozupone, C. A. et al., Meta-analyses of studies of the human microbiota. Genome Res., 2013, 23, 1704–1714.
- Caporaso, J. G. et al., QIIME allows analysis of high-throughput community sequencing data. Nat. Methods, 2010, 7, 335–336.
- DeSantis, T. Z. et al., NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes. Nucleic Acids Res., 2006, 34, 394–399.
- DeSantis, T. Z. et al., Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl. Environ. Microbiol., 2006, 72, 5069–5072.
- Osundiya, O. O., Oladele, R. O. and Oduyebo, O. O., Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. Afr. J. Clin. Exp. Microbiol., 2013, 14, 164–168.
- Loui, C., Grigoryan, G., Huang, H., Riley, L. W. and Lu, S., Bacterial communities associated with retail alfalfa sprouts. J. Food Protect., 2008, 71, 200–204.
- Sergeant, M. J., Constantinidou, C., Cogan, T. A., Bedford, M. R., Penn, C. W. and Pallen, M. J., Extensive microbial and functional diversity within the chicken cecal microbiome. PLoS ONE, 2014, 9, e91941.
- Modak, A., Kumar, V. and Rao, K. V. B., Microbial quality assessment of broiler chicken meat and evaluation of antibiotic susceptibility profile of isolates from retail outlets of Vellore, Tamil Nadu, India. Res. J. Pharm. Biol. Chem. Sci., 2014, 5, 1195–1202.
- Diarrassouba, F., Diarra, M. S., Bach, S., Delaquis, P., Pritchard, J., Topp, E. and Skura, B. J., Antibiotic resistance and virulence genes in commensal Escherichia coli and Salmonella isolates from commercial broiler chicken farms. J. Food Protect., 2007, 70, 1316–1327.
- Saroj, S. D., Shashidhar, R., Dhokane, V., Hajare, S., Sharma, A. and Bandekar, J. R., Microbiological evaluation of sprouts marketed in Mumbai, India, and its suburbs. J. Food Protect., 2006, 69, 2515–2518.
- Bhandare, S., Paturkar, A. M., Waskar, V. S. and Zende, R. J., Prevalence of microorganisms of hygienic interest in an organized abattoir in Mumbai, India. J. Infect. Dev. Ctries., 2010, 4, 454–458.
- Phillips, I., The use of bacitracin as a growth promoter in animals produces no risk to human health. J. Antimicrob. Chemoth., 1999, 44, 725–728.
- Lalzampuia, H., Dutta, T. K., Warjri, I. and Chandra, R., PCRbased detection of extended-spectrum -lactamases (blaCTX-M-1 and blaTEM) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae isolated from pigs in North Eastern India (Mizoram). Indian J. Microbiol., 2013, 53, 291–296.
- Gad, G. F., Mohamed, H. A. and Ashour, H. M., Aminoglycoside resistance rates, phenotypes, and mechanisms of gram-negative bacteria from infected patients in upper Egypt. PLoS ONE, 2011, 6, e17224.
- Falkiner, F. R., The consequences of antibiotic use in horticulture. J. Antimicrob. Chemother., 1998, 41, 429–431.
- Sung, J. Y. and Oh, J.-E., Distribution and characterization of integrons in Enterobacteriaceae isolates from chickens in Korea. J. Microbiol. Biotechnol., 2014, 24, 1008–1013.
- Chandrakanth, R. K., Raju, S. and Patil, S. A., Aminoglycosideresistance mechanisms in multidrug-resistant Staphylococcus aureus clinical isolates. Curr. Microbiol., 2008, 56, 558–562.
- Huddleston, J. R., Zak, J. C. and Jeter, R. M., Antimicrobial susceptibilities of Aeromonas spp. isolated from environmental sources. Appl. Environ. Microbiol., 2006, 72, 7036–7042.
- Stanley, D., Denman, S. E., Hughes, R. J., Geier, M. S., Crowley, T. M., Chen, H., Haring, V. R. and Moore, R. J., Intestinal microbiota associated with differential feed conversion efficiency in chickens. Appl. Microbiol. Biotechnol., 2012, 96, 1361–1369.
- Shaufi, M. A. M., Sieo, C. C., Chong, C. W., Gan, H. M. And Ho, Y. W., Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses. Gut Pathog., 2015, 7, 1–12.
- Leff, J. W. and Fierer, N., Bacterial communities associated with the surfaces of fresh fruits and vegetables. PLoS ONE, 2013, 8, e59310.
- Oakley, B. B. et al., The poultry-associated microbiome: network analysis and farm-to-fork characterizations. PLoS ONE, 2013, 8, e57190.
- Brandt, L. J., Kosche, K. A., Greenwald, D. A. and Berkman, D., Clostridium difficile-associated diarrhoea in the elderly. Am. J. Gastroenterol., 1999, 94, 3263–3266.
- Ahn, J. et al., Oralmicrobiome profiles: 16S rRNA pyrosequencing and microarray assay comparison. PLoS ONE, 2011, 6, e22788.
- Yang, F. et al., Saliva microbiomes distinguish caries-active from healthy human populations. ISMEJ, 2012, 6, 1–10.
- Yoon, E.-J. et al., Origin in Acinetobacter guillouiae and dissemination of the aminoglycoside-modifying enzyme Aph (3')-VI. mBio., 2014, 5, e01972-14.
- Bukhari, S. Z., Hussain, W. M., Fatani, M. I. and Ashshi, A. M., Multi-drug resistant Ewingella americana. Saudi Med. J., 2008, 29, 1051–1053.
- Wagner, M., Loy, A., Nogueira, R., Purkhold, U., Lee, N. and Daims, H., Microbial community composition and function in wastewater treatment plants. Antonie Leeuwenhoek, 2002, 81, 665–680.
- Sun, H. Y., Deng, S. P. and Raun, W. R., Bacterial community structure and diversity in a century-old manure-treated agroecosystem. Appl. Environ. Microbiol., 2004, 70, 5868–5874.
- Behravesh, C. B., Williams, I. T. and Tauxe, R. V., Emerging foodborne pathogens and problems: expanding prevention efforts before slaughter or harvest. In Improving Food Safety through a One Health Approach: Workshop summary, Institute of Medicine, Washington (DC), National Academies Press, US, 2012, pp 307–331.